Cornified cell envelope(CE) of human skin which have a role in the barrier function to protect the underlying tissues in dry condition. Human oral keratinocytes is in wet condition as saliva containing many proteases, growth factors, and many kinds of bacteria. Protective barrier of oral epithelium would be different from that skin epithelium barrier. Surface cell study of normal oral mucosa and oral mucosal lesions was restricted to examine the morphologic features by SEM and TEM. There is no reported about in vitro study on CE of normal human oral keratinocytes. The purpose of this study was to examine the morphology, and to analysis the amino acid component in CE of cultured human normal oral keratinocyte. It will be helpful to study oral mucosal diseases through amino acid component analysis of CE.
1. Both group showed large areas of statification, more compact, with irregular border and tightly apposed cells in 1.2mM.
2. Cornified cell envelope exhibited a fairly regular pattern of microridges. Anastmotic ridges forming pits and microridges were arranged mostly in parallel rows with frequent branches.
3. During the terminal differentiation in cultured NHEK and NHOK, insoluble cornified cell envelope formation was increased.
4. Cultured NHEK showed Gln/Glu(Involucrion), Gly(Loricrin), and Serine in descending over, while cultured NHOK showed Pro(SPR), Gln/Glu(Involucrion), Gly(Loricrin) in descending order.
From the aboving results, it was sugested that although during the terminal differentiation in cultured NHEK and NHOK, insoluble cornified cell envelope formation was increased, major amino component of cultured NHEK(Gln/Glu) was different from that of cultured NHOK(Pro).
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